It's usual for disinfecting contaminated worms as well as for synchronizing worms for large scale worm plating.

1. Wash the worms off the plate with M-9 Buffer. Use a pastuer pipette to squirt the worms off the plate and then transfer the worm suspension into a sterile 50ml screw cap tube.

2. Pellet the worms at 1/2 speed in a microcentrifuge for 2 minutes.

3. Aspirate and discard the supernatant. Add 10 ml of Chlorox/NaOH Solution (1 part Chlorox/3.5 parts of 0.625N NaOH) per ml of packed worms. (If your volume of packed worms is less than 1 ml, use 10 ml of the Chlorox/NaOH Solution).

4. Vortex vigorously for 3 minutees for complete decontamination. Spin down the worms as before.

5. Aspirate the supernatant and resuspend in 40 ml of M-9 Buffer. Spin down the worms as before.

6. Aspirate the supernatant and resuspend the pellet in a minimum amount of M-9 Buffer.

7. Plate the suspension on fresh plates with OP-50 bacteria and allow the eggs to hatch into worms.

As you can see, you are going to need two buffers that you don't have. If you like, I can prepare those buffers for you to pick up on your next visit to the Waksman building.